The instructions on how to add a persistence file are included inside each of the .mnu files. This is just a grub4dos menu which will be read by Easy2Boot during startup and the contents will be added to it's own 'in-memory' menu. However, when booting from a USB drive with some linux ISOs, Easy2Boot can boot directly from the ISO and enable persistence.
When you boot from a CD or DVD, persistence is not normally enabled because a CD/DVD does not support 'random-writes'.